Welcome to Protein World!. Protein Chemistry in 18th Century Organic Chemistry Achievements: Wheat Gluten (Jacob Bekkary) - 1728 Egg albumin (K. Neyman) - презентация

1 Welcome to Protein World!

2 Protein Chemistry in 18th Century Organic Chemistry Achievements: Wheat Gluten (Jacob Bekkary) Egg albumin (K. Neyman) Proteins are plant and animal origin organic substances with following properties: coagulation, glue, elastic, viscous and after dry retorting they emit gaseous ammonia

3 Protein Chemistry in 19th Century Organic Chemistry Achievements: Acidic protein hydrolysis (Brakkonne) (The protein chemistry Birth year) It was shown that all proteins have the same composition It was proposed the terminology Protein (Mulder) (It means primary in Greece-proteios)

4 Protein Chemistry in 20th Century Multidiscipline Science Achievements: Emil Fisher postulates (1900) 1. -Amino Acids 2.L-Amino Acids 3.Bound by peptide bonds 4.Polymers Diketopiperazine protein structure vs. peptide one ?

5 Diketopiperazine protein theory

6 Protein Chemistry in 20th Century Edman Peptide Degradation (sequencing) Protein secondary structure (Pauling and Corey)-1951 Insulin Primary Structure (Sanger) Amino Acid analyzer (Moore & Stain) Protein purification methods Protein X-Ray analysis (protein crystals) Electron Microscopy (x 50,000) C. Anfinsen Protein Assembling (Nobel Prize) Nucleic Acid sequencing

7 Protein Chemistry as a part of Organic Chemistry Vitamin B 12 Tryptophan

8 INSULIN

9 Insulin natural complex

10 Protein stability classification Thermostability High pressure stability Digestion stability Oxidative stability Shelf life. Microbial stability Chemical modification stability Protein immobilization and stability What is common one in the various types of the stability?

11 Методы выделения и исследования биологических соединений Структура – Свойства – Метод выделения

12 Что такое химия белка? Химия аминокислот Химия пептидов Химия белков