Строение ДНК Аксянов Е.А., 2008 ФББ МГУ 1. A-, B-DNA 2. - презентация

1 Строение ДНК Аксянов Е.А., 2008 ФББ МГУ 1

2 A-, B-DNA 2

3 УК-пары 3 Кто может сам нарисовать? Или хотя бы узнать их на картинке?

4 Взаимодействия с белками 4

5 Unusual topology srtuctures Slipped strands – Important for the tandem instability Cruciform Hollyday structures other 5

6 Slipped strands Neurodegenerative diseases: 1.Myotonic dystrophy types 1 and 2 (DM1 and DM2) 2.Fragile X syndrome 3.Friedreich ataxia 4.Spinocerebellar ataxia type 10 (SCA10). In SCA10 and DM2, expansion occurs to 4,500 and 11,000 repeats, respectively.

7 Первая структура ДНК в деталях 1: Nature Dec 13;282(5740):680-6.Links Molecular structure of a left-handed double helical DNA fragment at atomic resolution. Wang AH, Quigley GJ, Kolpak FJ, Crawford JL, van Boom JH, van der Marel G, Rich A. Wang AHQuigley GJKolpak FJCrawford JLvan Boom JHvan der Marel GRich A The DNA fragment d(CpGpCpGpCpG) crystallises as a left-handed double helical molecule with Watson-Crick base pairs and an antiparallel organisation of the sugar phosphate chains. The helix has two nucleotides in the asymmetric unit and contains twelve base pairs per turn. It differs significantly from right-handed B-DNA. PMID: [PubMed - indexed for MEDLINE] 7

8 Z-DNA 8 Редактирование mRNA рецептора глутамата: Q (CAG) -> R (CGG)

9 Z-DNA 9

10 Hoogsteen and RH interactions 10

11 H-DNA 11

12 TFO as drugs 1.Site-specific inhibition 2.Directed mutagenesis 3.Initiation of rearrangements 12

13 G-quartet 13

14 G-quartet, Z-DNA: regulation of expression c-MYC 14 Z-DNA

15 15

16

17

18 Топология: сверхспирализация 18

19 Топология 19

20 Топология: репликация 20

21 Пока все